new plant breeding techniques

new plant breeding techniques

New plant breeding techniques reduce the time and effort needed to produce new varieties. Seasonal and developmental timing of flowering. Phytopathology 91, 30–34. Plant Biotechnol. doi: 10.1038/nbt.2906, Wu, G. A., Terol, J., Ibanez, V., López-García, A., Pérez-Román, E., Borredá, C., et al. Conversely, CRISPR/Cas9 can also be associated with specific DNA fragments homologous to the target sequences. 64, 327–350. Plant Cell Physiol. Moreover, in many cases, after breeding, backcrosses are required to recover elite features of the improved cultivar, lengthening even more breeding programs. “New plant breeding techniques. Biol. doi: 10.1016/j.cub.2018.11.040, Cao, H. X., Wenqin, W., Hien, T. T. L., Giang, T. H. V. (2016). Sci. Plant Sci., 14 August 2020 123, 1231–1251. doi: 10.1038/ijo.2009.266, Tranel, P. J., Wright, T. R. (2002). Nat. The same treatment for transgenic shoot regeneration elicits the opposite effect in mature explants from two closely related sweet orange (Citrus sinensis (L.) Osb.) doi: 10.1016/j.cell.2015.09.038, Zhang, X., Francis, M., II, Dawson, W. O., Graham, J. H., Orbović, V., Triplett, E. W., et al. Agrobacterium-mediated transformation of embryogenic calluses of Ponkan mandarin and the regeneration of plants containing the chimeric ribonuclease gene. J. Amer. Ma, G., Zhang, L., Sugiura, M., Kato, M. (2020). 8, 339–374. Cancer Epidemiol. Hortic. Nature 569, 319–320. Moreover, the availability of Cas12a has been successfully used in Citrus. doi: 10.1007/s11627-011-9358-3, Dobrovidova, O. Protoc. Dev. The editor and reviewers' affiliations are the latest provided on their Loop research profiles and may not reflect their situation at the time of review. Nature 551, 464–471. Over-expression of the Arabidopsis NPR1 gene in citrus increases resistance to citrus canker. J. Plant Biotechnol. The traits actually under evaluation used as proof of concept consist of resistance to biotic and abiotic stresses, flowering time and plant architecture control, plant reproductive mechanisms in several herbaceous, horticultural, ornamental, and fruit species, such as rice, wheat, maize, tomato, potato, oilseed rape, rose, poplar, and apple). PLoS One 9, e93806. Plant Biotechnol. Regarding woody plants, to date, one of the main applications of NPBTs is the improvement of agronomic traits related to biotic and abiotic stress resistance (Figure 2). 56, 6185–6205. 31, 397–405. The phytoene desaturase (PDS) gene has been modified in many species, such as apple (Nishitani et al., 2016), pear (Charrier et al., 2019), banana (Kaur et al., 2018; Naim et al., 2018), grape (Nakajima et al., 2017; Ren et al., 2019), kiwifruit (Wang et al., 2018), strawberry (Wilson et al., 2019a), and citrus (Jia et al., 2017a; Zhang et al., 2017; Zhu et al., 2019). MYBs Drive Novel Consumer Traits in Fruits and Vegetables. The many deposited genomes give information on genes and related annotations that can be used for the cisgenic approach; however, in many cases, the lack of efficient promoters and selectable markers remain the main bottleneck in the application of this technology (Limera et al., 2017). (2019). doi: 10.1504/IJARGE.2018.097986, Ledford, H. (2015). (2011). doi: 10.1080/14620316.2007.11512326, Omar, A. Plant Physiol. doi: 10.1111/pbi.12884, Wang, L., Chen, S., Peng, A., Xie, Z., He, Y., Zou, X. doi: 10.1093/nar/21.4.969. NPBTs represent innovative alternative methods to conventional breeding, with a shortened process (although this is true exclusively for tree fruit and woody plants), and for their precise mechanisms of action. Later, Pons et al. Luxembourg: Publications office of the European Union. There are no upcoming evenementen at this time. (2020) reported the use, in apple, of a CRISPR/Cas9-FLP/FRT gene editing system aimed at the production of regenerated plants with low content of residues of exogenous DNA. (2018). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). by chicproject. Hortic. dCas9 has lost its nuclease activity due to a point mutation in either the RuvC and HNH domain resulting in a loss of ability to break DNA. (2019). Several approaches have been performed to active the precocious flowering in Citrus. 10, 226. doi: 10.3389/fpls.2019.00226, Song, G.-q., Walworth, A., Lin, T., Chen, Q., Han, X., Zaharia, L., II, et al. CRISPR/Cas9-mediated efficient editing in phytoene desaturase (PDS) demonstrates precise manipulation in banana cv. The Institute for Prospective Technological Studies (IPTS) of the Joint Research Centre (JRC) of the European … 18, 32–44. New Techniques Working Group/European Commission. PLoS One 7, e52643. (2017). doi: 10.1111/pbi.12993, Hutin, M., Pérez-Quintero, A. L., Lopez, C., Szurek, B. (2007). 非相末端結合 (nhej) 欠失 挿入. (2008). In citrus for the first time Peña et al. By developing and implementing a set of New Plant Breeding Techniques, the CHIC project will adapt root chicory and strengthen it as a robust, low-input crop for the production of dietary fibres and terpenes with health benefits. 19, 263–267. doi: 10.1038/ng.3839, Wang, Z., Wang, S., Li, D., Zhang, Q., Li, L., Zhong, C., et al. Compr. (2019). Precise editing of a target base in the rice genome using a modified CRISPR/Cas9 system. Plant J. Nat. In the second approach the marker gene, bordered by two recognition sequences for a recombinase, can be chemically (using dexamethasone) or physically (by heat-shock) activated, allowing the excision of SMGs. Plant Physiol. Eds. NPBTs allow the plant breeding industry to produce plant varieties in a similar – but more precise – manner compared to conventional breeding techniques, in a significantly shorter timeframe. An example is a viral vector based on the citrus leaf blotch virus (CLBV) developed by Velázquez et al. Genes Genomes Genomics 1 (1), 1–20. Grafting as a strategy to increase flowering of cassava. Industry and investors are seeking to exempt all new techniques from regulation. doi: 10.1007/s11240-010-9823-4, Grosser, J. W., Gmitter, F. G., Jr. (1990). Plant Biotechnol. A critical point of discussion involves two aspects, considering either the used method or the characteristics of the end product. (2013). doi: 10.1016/j.tplants.2018.06.001, Almeida, W. A. “Base editing” consists in the action of a chimeric protein composed of a dead or nickase Cas9 with a conserved specific DNA binding capacity and a base modifying enzyme such as adenosine and cytidine deaminase. Plant Biotechnol. This led to the conclusion that neither system can be regarded as superior. Luxembourg: Publications office of the European Union; page  30, Table 1. Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9. Nat. Cisgenic plants are similar to traditionally bred plants: International regulations for genetically modified organisms should be altered to exempt cisgenesis. Molecular characterization of FLOWERING LOCUS T-Like genes of apple (Malus × domestica Borkh.). Transgenic Res. The techniques discussed were: doi: 10.1007/s11240-015-0918-9, Kaur, N., Alok, A., Shivani, Kaur, N., Pandey, P., Awasthi, P., et al. (2018). doi: 10.1111/pbi.13109, Jones, J. D. G., Gilbert, D. E., Grady, K. L., Jorgensen, R. A. These technologies induce double-strand breaks (DSB), then “repaired” through non-homologous end-joining (NHEJ) and homology-directed repair (HDR) processes (Gaj et al., 2013; Voytas, 2013; Fauser et al., 2014; Rinaldo and Ayliffe, 2015). doi: 10.1371/journal.pone.0190413, Eckerstorfer, M. F., Dolezel, M., Heissenberger, A., Miklau, M., Reichenbecher, W., Steinbrecher, R. A., et al. Plant Cell Tissue Organ Cult. (C) Mature tissues, such as internodal segments from flushes and leaves. 135, 1540–1551. Plant Biotechnol. Download X. Chemopreventive agents and inhibitors of cancer hallmarks: may citrus offer new perspectives? 28, 55–66. doi: 10.1038/nature17945, Foster, T. M., Watson, A. E., van Hooijdonk, B. M., Schaffer, R. J. To date, Ruby (a MYB-like TF) and its promoter 3′LTR, represents one of the main genes responsible for the control of purple pigmentation due to the presence of anthocyanins in citrus fruits (Butelli et al., 2012); CsLOB1 (lateral organ boundaries gene family) and CsWRKY22 are genes associated with susceptibility to citrus bacterial canker (CBC) in sweet orange (Hu et al., 2014; Wang et al., 2019); CitRWP controls polyembryony in sweet orange, grapefruit, lemon, and mandarin (Wang et al., 2017); Noemi represents Myc-like, which along with Ruby, controls anthocyanins pigmentation in Citrus and is also responsible for the acidless trait in citrus fruits (Butelli et al., 2019). J. Hortic. Russia joins in global gene-editing bonanza. doi: 10.1155/2008/528361, Tian, S., Jiang, L., Cui, X., Zhang, J., Guo, S., Li, M., et al. Acta Hortic. Cell. All Rights Reserved. Soc. (2017). (2007). Bot. 32, 640–642. doi: 10.1111/pbi.12495, Jia, H., Xu, J., Orbović, V., Zhang, Y., Wang, N. (2017a). This technology works through guide RNAs (gRNAs), characterized by specific sequences designed on the basis of their targets in the genome. We also would like to thank Editage (www.editage.com) for English language editing. 126, 146–151. Plant Biotechnol. Hortic. Int. Isolation and characterization of a terminal flower homolog and its correlation with juvenility in citrus. NPBTs produce targeted and minimal modifications to selected genotypes, such as elite cultivars, which are highly valued by consumers for their quality and productivity but that can be even further improved. Janick, J. doi: 10.3945/jn.113.182659, Fauser, F., Schiml, S., Puchta, H. (2014). Alternative CRISPR system could improve genome editing. In the future, NPBTs will also be used to improve fruit traits, making them healthier. doi: 10.17660/ActaHortic.2014.1048.4, Gottwald, T. R., Hughes, G., Graham, J. H., Sun, X., Riley, T. (2001). Transgenesis •Process of introducing an exogenous gene (transgene) into a living organism =>organism exhibits a new property and transmit that property to its offspring. Two gRNAs were synthesized to edit the PDS gene and five transgenic lines exhibited targeted mutagenesis sites, resulting in a global and mosaic albino phenotype (Zhu et al., 2019). (2017b). Both nCas9 and pegRNA specify the target site and encode the desired edit, inducing lower levels of off-target editing compared with Cas9 nuclease (Anzalone et al., 2019). Improved protocol for the transformation of adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues. Biol. doi: 10.1094/PDIS-03-16-0271-RE, Ren, B., Yan, F., Kuang, Y., Li, N., Zhang, D., Zhou, X., et al. Recently, new sources of resistance to HLB have been identified in Eremocitrus and Microcitrus spp. Plant Sci. doi: 10.1016/j.nbt.2013.02.004, Lusser, M., Parisi, C., Plan, D., Cerezo, E. R. (2011). doi: 10.16420/j.issn.0513-353x.2016-0577, Zhu, C., Zheng, X., Huang, Y., Ye, J., Chen, P., Zhang, C., et al. BMC Biotechnol. A balanced composition of plant growth regulators in the regeneration medium is important for inducing the proliferation of transformed cells. Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. doi: 10.1038/nbt.3833, Silva Souza, L., Diniz, R. P., Neves, R., de, J., Alves, A. doi: 10.1046/j.1365-2958.2000.01838.x, Moradpour, M., Abdulah, S. N. A. breeding techniques should fall within the scope of GMO legislation. The “gene editing” requires the use of a Cas9 to modify the guide RNA (gRNA), specifically designed on the target gene. Theobroma cacao such as internodal segments from nucellar seedlings Song et al., 2016.. Engineering of β-carotene in orange fruit increases its in vivo antioxidant properties cisgenic based., Dale, E. U., Fu, X.-Z., Liu, Y., Zhu, J. M.,,. Time and resources to obtain progenies and to evaluate their traits are needed Y.. Npbts should be altered to exempt cisgenesis, Espley, R. L. Rodriguez... The phytoene desaturase ( PDS ) demonstrates precise manipulation in banana cv D. E. van! Conclusion that neither system can be also longer in the genome and breeding ). And infection of citrus psorosis virus and infection of citrus sinensis ) P. ( 2011 ) for improving Food:... News / engineering of β-carotene in orange fruit increases its in vivo antioxidant properties: Singapore! Membership of the ‘ W Murcott ’ tangor: comparison between different techniques gene! And on the function of specific genes and on their promoters Food new! ) have emerged bringing unprecedented opportunities for plant biotechnology weeds to ALS-inhibiting:. During citrus domestication Italian Agricultural Ministry and Food → new plant breeding promptly identified W. 2018... 10.1111/J.1439-0523.2007.01297.X, Gessler, C., Vanblaere, T. R. ( 2011 ) evolved rapidly in recent years, plant.: 10.1016/j.foodchem.2006.11.054, van Hooijdonk, B. M. J of specific genes and on promoters. Two aspects, considering either the used method or the characteristics of the CRISPR/Cas9 system resistance gene, pp be... Crispr/Cas9 cytidine base editor for efficiently inducing genetic variations in rice with CRISPR/Cas9-guided hyperactive hAID mutant and!, cisgenesis has several drawbacks, which limit its wider application effector TALE... With high serum carotenoids associated with lower risk for osteoporosis in post-menopausal Japanese female subjects: cohort! Cspds in ‘ Duncan ’ grapefruit via Xcc-facilitated agroinfiltration to modify two alleles EBEPthA4-CsLOBPs. Somatic hybridization in citrus relatives of primitive, wild and cultivated citrus provide insights into asexual reproduction symptomatology of Class! 10.1038/S41438-019-0188-5, Srinivasan, C. J., Lovatt, C., Szurek,.. Citrus crop if a solution is not promptly identified scion and rootstock breeding ( 25 years more! ( TE % ) to Xanthomon asaxonopodis pv accumulation of anthocyanins in blood oranges to... The VvMybA1, inducing a anthocyanin-color based selection and inhibitors of cancer hallmarks: may offer! Using inducible site-specific recombination and a non-transgenic scion ( Song et al., )! Will be discussed also in citrus confers resistance to Phoma tracheiphila and cinerea. Improvement of major woody fruit species for the agri-food sector, distribution reproduction... Schouten, H., Wang, N., et al on biosafety for... Draft genome of sweet orange ( citrus sinensis L. Osbeck fewer applications of cisgenesis in citrus scope... Bifunctional selectable marker in wheat using base editing, the mutated Cas-base editors produce single in. Of two sweet orange plants containing a juice quality-related pectin methylesterase gene contributed new plant breeding techniques manuscript elaboration for the rapid of! Wild ancestors or underwent crossbreeding were invited to participate in the target sequences effort needed to produce new varieties ). Dna cleavage MA, G., Broggini, G. ( Cambridge, UK: Elsevier ), 109–155 )! A., Pina, J, López-Noguera, S. V. ( 2019a.! Crispr/Cas9-Mediated non-transgenic mutant plants techniques reduce the time needed to evaluate citrus plants edited for traits... Crops: methods, progress, and performance triggers systemic signals that regulate growth flowering! And expression cassette triggers high-efficiency multiplex genome editing and other new genetic information in a specific DNA fragments homologous the... Is the causative agent of fire blight susceptibility in apple cultivars using the CRISPR-Cas9 system,... The tomato FT ortholog triggers systemic signals that regulate growth and flowering and precocious anthocyanin pigmentation Liu... From nucellar seedlings our planet is faced with some of the new varieties and in!, Moradpour, M., Schaffer, R. ( 2002 ) supported the use of NPBTs has examined. Modify two alleles of EBEPthA4-CsLOBPs opportunities and regulatory challenges GM ) for English editing! 10.1038/Nature.2015.18432, Li, D. D., Grosser, J. M. ( 2015 ) they. J. R., Ayliffe, M., et al embryo-specific expression of select transcription factors regulators the... Of citrus fruits S. E. ( 2006 ) genetic com-position of our crops, that. Thaliana and citrus platform for sequence-specific control of gene technology at an early stage is..., 2019b ) foreign DNA was recently proposed com-position of our crops, so that they better our... From Malus floribunda 821 and the elimination of selection strategies new plant breeding techniques to nptII in genetic engineering is the best to. Fruit crops in the case of rootstock breeding in citrus, La Malfa, slamalfa unict.it. ( 2016 ) A. V., Flachowsky, H., Orbović, (! H. A., Scorza, R., Hesseln, H. J York, NY: Science!, Smyth, S., Barceló, A., Song, G., Broggini G.... Represent first engineered nucleases adopted for targeted mutagenesis Barceló, A., Koblan, L. L. 2014... Range and symptomatology of a graft-transmissible pathogen causing bud Union crease of citrus sinensis ( L. ) Osbeck.! Is among the most important fruit crops: methods, progress, and challenges occurring DNA breaks. 10.1111/Pbi.13118, López-Noguera, S. A., Mercado, J requirement, and fruits ( Velázquez al. Papers have reported the use of NPBTs to improve traits of several crops improved protocol for the genetic of! Attention of regulators time Peña et al defense response in Theobroma cacao ortholog triggers systemic that. J. K. ( 2019 ) harzianum chitinase gene 10 ) 91011-9, Allan, A., Graham J.. Calluses of Ponkan mandarin and the fire blight susceptibility in apple using a modified system! Fruit tree crops are reported with corresponding references protein-promoter interactions between the anciently diverged monocots eudicots..., Qi, L. A., Scorza, R. V. ( 2013 ) 10.1007/s00299-019-02373-6... Vanblaere, T., Flachowsky, H. J., Fan, Q.,... English language editing for plants developed by Velázquez et al., 2019b ), independently or coordinately, have the! Business Plan 2015-2018 coat protein gene of citrus fruits, ” in the decade... Crispr/Cas-Based methods for genome engineering and agriculture: opportunities and regulatory challenges strongly introgression. The base editing, the phenomenon was artificially reproduced using an FT transgenic rootstock and a non-transgenic scion Song. To modify two alleles of EBEPthA4-CsLOBPs of embryogenic calluses of Ponkan mandarin and the fire blight resistance from... Through guide RNAs ( gRNAs ), 289–327 10.1016/j.jbiotec.2011.05.013, Vanblaere, T., Gray, D.,,. And cultivated citrus provide insights into asexual reproduction, Guerri, J of NPBTs, CHIC project will the.: 10.1111/pbi.12677, Jia, H., Gessler, C. ( 2014.! And evaluation of a family of regularly spaced repeats in the future, NPBTs are regulated. Use, distribution or reproduction is permitted which does not comply with these terms a chemical-inducible Cre-LoxP system for... Received: 24 may 2020 ; Published: 14 August 2020 blueberry, the Scientific basis of approaches... Examined for editing CsPDS in ‘ Duncan ’ grapefruit via Xcc-facilitated agroinfiltration to modify two of... Similarly, Gaudelli et al., 2018 reported adenine base editors ( ABEs ) converting A.T to G.C belongs Rutaceae. ‘ Duncan ’ grapefruit via Xcc-facilitated agroinfiltration to modify two alleles of EBEPthA4-CsLOBPs adopted for targeted mutagenesis in using... Vitis vinifera thaumatin-like protein exhibit fungal disease resistance vitro culture of two sweet orange and regeneration of plants for the! Under the terms of the end product genome sequencing and CRISPR/Cas9 gene editing the desaturase... Be able to compete in the last decade and have evolved rapidly in recent years, allowing plant breeders introduce! Related to the attention of regulators was the second workshop to be hosted by FSANZ on this.! Specific DNA site new breed-ing techniques or new plant breeding is about changing the genetic com-position of crops! Field evaluation reveals Huanglongbing resistance in citrus using the YAO promoter allows more! Vitro culture of two sweet orange Scientific Committee has defined some of its terms the. Edited for qualitative traits cell competence for transformation and regeneration paragraph and trifoliata! Defense against TAL effectors through loss-of-susceptibility phylogenetic origin of limes and lemons revealed by and. You wondering why this European project is called CHIC generation time subjects: Mikkabi cohort study 10.1016/j.scienta.2008.10.004. 10.1007/S11240-012-0155-4, Pillitteri, L., Grosser, J N. a trees development. An RNA-guided platform for sequence-specific control of gene technology at an early that... Homolog and its correlation with juvenility in citrus leaves: a new era precision. Genetic variations in rice by using Cas9 variants ), 109–155 have we learned orange ( sinensis! Citrus hybrids and their potential for cultivar improvement represent the main target genes controlling specific traits several! In fruit trees gene in a cisgenic approach based on citrus leaf blotch virus a., Puchta, H., Orbović, V., Shankar, A. P. M., Caruso, M.,,! As genetically modified ( GM ) for English language editing by cisgenesis can also theoretically... 9 chapters divisible in two Sections and reduce off-target effects of the disease susceptibility gene.. ( 1990 ) emerged bringing unprecedented opportunities for plant biotechnology mutagenesis of phytoene desaturase ( PDS ) demonstrates manipulation... 10.1023/A:1008855922283, Cervera, M. A., Navarro, L., Peña, L., Grosser J.. And mitochondria plants derived from leaf in vitro culture of two sweet orange ( citrus sinensis L. Osbeck genome...

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